Quantitative ratio of mRNA expression of IGF/INS system receptors in multiple myeloma

Background. Individuals with increased expression of components the IGF/INS system, are more likely to develop various malignancies. And in case when system overexpressed tumors, this adversely affects prognosis disease, including leading a decrease relapse-free survival. A characteristic feature is ability same ligands bind different receptors and vice versa (cross interactions) activate signaling pathways cell. This requires an integrated approach study its components, namely, quantitative ratio individual components. The result obtained will make it possible determine combinations ligand-receptor bonds and, ultimately, have both prognostic evaluative value: terms therapeutic target. Aim. To establish mRNA receptors: IR-A, IR-B, IGF-1R, IGF-2R IM9 lymphoblastoid cell line three myeloma lines: RPMI1640, RPMI8226, H929, identify frequency these mononuclear fraction bone marrow aspirates from treated patients multiple myeloma. Materials methods. We used human cells 3 types cells, differing degree differentiation as well 19 stage III Expression was studied by real-time reverse transcription polymerase chain reaction aspirate samples semi-quantitative reaction. Results. During study, we found that within each line, receptor IR-A predominant compared IR-B . Patients MM high minimum IGF-1R:IR-A IGF-1R:IR-B for ratios high. IGF-2R:IR-A maximum or times less. Conclusion. Based on mRNA, state there probability presence IGF-1R/IGF-1R IR-A/IR-A homodimers, IGF-1R/IR-A heterodimer. These data value, since suggest significant increase mitogenic effect due activation ligands: IGF-1, IGF-2 INS, which unfavorable factor, especially patient concomitant Diabetes mellitus prescribed insulin therapy along chemotherapy. our findings, recommend simultaneously inhibiting IGF-1R